Construct: shRNA TRCN0000311707

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_004044.5-476s21c1
DNA Barcode:
GCAATCTCTATCCCTTTGTAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
ATIC (471)
Gene Description:
5-aminoimidazole-4-carboxamide ribonucleotide formyltransferase/IMP cyclohydrolase
Transcript:
RefSeq NM_004044.5 (NON-CURRENT)
Match location:
Position 476 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000311707)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCAATCTCTATCCCTTTGTAA
Hairpin Sequence:
5'-CCGG-GCAATCTCTATCCCTTTGTAA-CTCGAG-TTACAAAGGGATAGAGATTGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCAATCTCTATCCCTTTGTAACTCGAGTTACAAAGGGATAGAGATTGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCAATCTCTATCCCTTTGTAACTCGAGTTACAAAGGGATAGAGATTGC-3'

Other clones with same target sequence:

  1. TRCN0000034967