Construct: shRNA TRCN0000311848

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_016791.3-1985s21c1
DNA Barcode:
CCCGTCCAAGTCAGTTTCTAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Nfatc1 (18018)
Gene Description:
nuclear factor of activated T cells, cytoplasmic, calcineurin dependent 1
Transcript:
RefSeq NM_016791.3 (NON-CURRENT)
Match location:
Position 1985 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000311848)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCCGTCCAAGTCAGTTTCTAT
Hairpin Sequence:
5'-CCGG-CCCGTCCAAGTCAGTTTCTAT-CTCGAG-ATAGAAACTGACTTGGACGGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCCGTCCAAGTCAGTTTCTATCTCGAGATAGAAACTGACTTGGACGGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCCGTCCAAGTCAGTTTCTATCTCGAGATAGAAACTGACTTGGACGGG-3'

Other clones with same target sequence:

  1. TRCN0000081925