Construct: shRNA TRCN0000311870

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_019778.1-1735s21c1
DNA Barcode:
GCCTTCAGTCAACACATCCAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Zbtb20 (56490)
Gene Description:
zinc finger and BTB domain containing 20
Transcript:
RefSeq NM_019778.1 (NON-CURRENT)
Match location:
Position 1735 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000311870)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCCTTCAGTCAACACATCCAT
Hairpin Sequence:
5'-CCGG-GCCTTCAGTCAACACATCCAT-CTCGAG-ATGGATGTGTTGACTGAAGGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCCTTCAGTCAACACATCCATCTCGAGATGGATGTGTTGACTGAAGGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCCTTCAGTCAACACATCCATCTCGAGATGGATGTGTTGACTGAAGGC-3'

Other clones with same target sequence:

  1. TRCN0000086331