Construct: shRNA TRCN0000311895

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_133352.2-454s21c1
DNA Barcode:
CCAGGCACTTACTGTGAAATT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Tm9sf3 (107358)
Gene Description:
transmembrane 9 superfamily member 3
Transcript:
RefSeq NM_133352.2 (CURRENT)
Match location:
Position 454 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000311895)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCAGGCACTTACTGTGAAATT
Hairpin Sequence:
5'-CCGG-CCAGGCACTTACTGTGAAATT-CTCGAG-AATTTCACAGTAAGTGCCTGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCAGGCACTTACTGTGAAATTCTCGAGAATTTCACAGTAAGTGCCTGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCAGGCACTTACTGTGAAATTCTCGAGAATTTCACAGTAAGTGCCTGG-3'

Other clones with same target sequence:

  1. TRCN0000124990