Construct: shRNA TRCN0000311931

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_026360.2-1472s21c1
DNA Barcode:
CCTCGGGATTGCAGTGTCATT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Ddx47 (67755)
Gene Description:
DEAD (Asp-Glu-Ala-Asp) box polypeptide 47
Transcript:
RefSeq NM_026360.2 (NON-CURRENT)
Match location:
Position 1472 (3UTR)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000311931)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCTCGGGATTGCAGTGTCATT
Hairpin Sequence:
5'-CCGG-CCTCGGGATTGCAGTGTCATT-CTCGAG-AATGACACTGCAATCCCGAGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCTCGGGATTGCAGTGTCATTCTCGAGAATGACACTGCAATCCCGAGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCTCGGGATTGCAGTGTCATTCTCGAGAATGACACTGCAATCCCGAGG-3'

Other clones with same target sequence:

  1. TRCN0000071098