Construct: shRNA TRCN0000311947

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_009786.1-248s21c1
DNA Barcode:
GCAACAGAAGTCGCAGAAGAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Cacybp (12301)
Gene Description:
calcyclin binding protein
Transcript:
RefSeq NM_009786.1 (NON-CURRENT)
Match location:
Position 248 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000311947)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCAACAGAAGTCGCAGAAGAA
Hairpin Sequence:
5'-CCGG-GCAACAGAAGTCGCAGAAGAA-CTCGAG-TTCTTCTGCGACTTCTGTTGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCAACAGAAGTCGCAGAAGAACTCGAGTTCTTCTGCGACTTCTGTTGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCAACAGAAGTCGCAGAAGAACTCGAGTTCTTCTGCGACTTCTGTTGC-3'

Other clones with same target sequence:

  1. TRCN0000125176