Construct: shRNA TRCN0000312037

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_016739.3-455s21c1
DNA Barcode:
GCCGTATCTAAGTACCAGGAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Caprin1 (53872)
Gene Description:
cell cycle associated protein 1
Transcript:
RefSeq NM_016739.3 (CURRENT)
Match location:
Position 455 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000312037)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCCGTATCTAAGTACCAGGAA
Hairpin Sequence:
5'-CCGG-GCCGTATCTAAGTACCAGGAA-CTCGAG-TTCCTGGTACTTAGATACGGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCCGTATCTAAGTACCAGGAACTCGAGTTCCTGGTACTTAGATACGGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCCGTATCTAAGTACCAGGAACTCGAGTTCCTGGTACTTAGATACGGC-3'

Other clones with same target sequence:

  1. TRCN0000102222