Construct: shRNA TRCN0000312052

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_172120.3-1185s21c1
DNA Barcode:
CGACATAAGATTCTGGATATT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Vps41 (218035)
Gene Description:
VPS41 HOPS complex subunit
Transcript:
RefSeq NM_172120.3 (NON-CURRENT)
Match location:
Position 1185 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000312052)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CGACATAAGATTCTGGATATT
Hairpin Sequence:
5'-CCGG-CGACATAAGATTCTGGATATT-CTCGAG-AATATCCAGAATCTTATGTCG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCGACATAAGATTCTGGATATTCTCGAGAATATCCAGAATCTTATGTCGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACGACATAAGATTCTGGATATTCTCGAGAATATCCAGAATCTTATGTCG-3'

Other clones with same target sequence:

  1. TRCN0000111607