Construct: shRNA TRCN0000312105

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_009367.3-3314s21c1
DNA Barcode:
CGGTGAAATGTTGACCTGTTT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Tgfb2 (21808)
Gene Description:
transforming growth factor, beta 2
Transcript:
RefSeq NM_009367.3 (NON-CURRENT)
Match location:
Position 3314 (3UTR)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000312105)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CGGTGAAATGTTGACCTGTTT
Hairpin Sequence:
5'-CCGG-CGGTGAAATGTTGACCTGTTT-CTCGAG-AAACAGGTCAACATTTCACCG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCGGTGAAATGTTGACCTGTTTCTCGAGAAACAGGTCAACATTTCACCGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACGGTGAAATGTTGACCTGTTTCTCGAGAAACAGGTCAACATTTCACCG-3'

Other clones with same target sequence:

  1. TRCN0000066093