Construct: shRNA TRCN0000312134

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_021430.2-1225s21c1
DNA Barcode:
CCAGATGGAGAGGAATCGATT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Rilpl1 (75695)
Gene Description:
Rab interacting lysosomal protein-like 1
Transcript:
RefSeq NM_021430.2 (CURRENT)
Match location:
Position 1225 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000312134)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCAGATGGAGAGGAATCGATT
Hairpin Sequence:
5'-CCGG-CCAGATGGAGAGGAATCGATT-CTCGAG-AATCGATTCCTCTCCATCTGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCAGATGGAGAGGAATCGATTCTCGAGAATCGATTCCTCTCCATCTGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCAGATGGAGAGGAATCGATTCTCGAGAATCGATTCCTCTCCATCTGG-3'

Other clones with same target sequence:

  1. TRCN0000220569