Construct: shRNA TRCN0000312140

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_013470.2-836s21c1
DNA Barcode:
CCGCAGCTGAAACTAACATTT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Anxa3 (11745)
Gene Description:
annexin A3
Transcript:
RefSeq NM_013470.2 (CURRENT)
Match location:
Position 836 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000312140)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCGCAGCTGAAACTAACATTT
Hairpin Sequence:
5'-CCGG-CCGCAGCTGAAACTAACATTT-CTCGAG-AAATGTTAGTTTCAGCTGCGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCGCAGCTGAAACTAACATTTCTCGAGAAATGTTAGTTTCAGCTGCGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCGCAGCTGAAACTAACATTTCTCGAGAAATGTTAGTTTCAGCTGCGG-3'

Other clones with same target sequence:

  1. TRCN0000110727