Construct: shRNA TRCN0000312163

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_010135.2-2840s21c1
DNA Barcode:
GAGCAAGTCGAACACTGCATA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Enah (13800)
Gene Description:
enabled homolog (Drosophila)
Transcript:
RefSeq NM_010135.2 (NON-CURRENT)
Match location:
Position 2840 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000312163)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GAGCAAGTCGAACACTGCATA
Hairpin Sequence:
5'-CCGG-GAGCAAGTCGAACACTGCATA-CTCGAG-TATGCAGTGTTCGACTTGCTC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGAGCAAGTCGAACACTGCATACTCGAGTATGCAGTGTTCGACTTGCTCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGAGCAAGTCGAACACTGCATACTCGAGTATGCAGTGTTCGACTTGCTC-3'

Other clones with same target sequence:

  1. TRCN0000091682