Construct: shRNA TRCN0000312206

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_009822.2-643s21c1
DNA Barcode:
GCAAGCTGAAACGATTTCTTA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Runx1t1 (12395)
Gene Description:
runt-related transcription factor 1; translocated to, 1 (cyclin D-related)
Transcript:
RefSeq NM_009822.2 (NON-CURRENT)
Match location:
Position 643 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000312206)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCAAGCTGAAACGATTTCTTA
Hairpin Sequence:
5'-CCGG-GCAAGCTGAAACGATTTCTTA-CTCGAG-TAAGAAATCGTTTCAGCTTGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCAAGCTGAAACGATTTCTTACTCGAGTAAGAAATCGTTTCAGCTTGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCAAGCTGAAACGATTTCTTACTCGAGTAAGAAATCGTTTCAGCTTGC-3'

Other clones with same target sequence:

  1. TRCN0000096595