Construct: shRNA TRCN0000312360

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_026002.4-1944s21c1
DNA Barcode:
CCCAAGTCAAATGCTAAGCAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Mtdh (67154)
Gene Description:
metadherin
Transcript:
RefSeq NM_026002.4 (CURRENT)
Match location:
Position 1944 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000312360)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCCAAGTCAAATGCTAAGCAA
Hairpin Sequence:
5'-CCGG-CCCAAGTCAAATGCTAAGCAA-CTCGAG-TTGCTTAGCATTTGACTTGGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCCAAGTCAAATGCTAAGCAACTCGAGTTGCTTAGCATTTGACTTGGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCCAAGTCAAATGCTAAGCAACTCGAGTTGCTTAGCATTTGACTTGGG-3'

Other clones with same target sequence:

  1. TRCN0000125815