Construct: shRNA TRCN0000312471

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_016033.2-777s21c1
DNA Barcode:
CTTCAATTCACCTTATGGGTA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
RMDN1 (51115)
Gene Description:
regulator of microtubule dynamics 1
Transcript:
RefSeq NM_016033.2 (NON-CURRENT)
Match location:
Position 777 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000312471)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CTTCAATTCACCTTATGGGTA
Hairpin Sequence:
5'-CCGG-CTTCAATTCACCTTATGGGTA-CTCGAG-TACCCATAAGGTGAATTGAAG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCTTCAATTCACCTTATGGGTACTCGAGTACCCATAAGGTGAATTGAAGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACTTCAATTCACCTTATGGGTACTCGAGTACCCATAAGGTGAATTGAAG-3'

Other clones with same target sequence:

  1. TRCN0000146666