Construct: shRNA TRCN0000312500
Construct Description:
- Construct Type:
- shRNA
- Other Identifiers:
- NM_025103.2-1594s21c1
- DNA Barcode:
- GACAACTGATCTGGAGATATA
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- IFT74 (80173)
- Gene Description:
- intraflagellar transport 74
- Transcript:
- RefSeq NM_025103.2 (NON-CURRENT)
- Match location:
- Position 1594 (CDS)
Vector Information:
- Vector Backbone:
- pLKO_005
- Pol II Cassette 1:
- PGK-PuroR
- Pol II Cassette 2:
- n/a
- Pol III Promoter:
- constitutive hU6
- Pol III Insert:
- (TRCN0000312500)
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
Current transcripts matched by this shRNA:
Sequence Information
- Target Sequence:
- GACAACTGATCTGGAGATATA
- Hairpin Sequence:
-
5'-CCGG-GACAACTGATCTGGAGATATA-CTCGAG-TATATCTCCAGATCAGTTGTC-TTTTTG-3'
Oligo design for arrayed cloning:
- Forward sequence:
- 5'-CCGGGACAACTGATCTGGAGATATACTCGAGTATATCTCCAGATCAGTTGTCTTTTTG-3'
- Reverse sequence:
- 5'-AATTCAAAAAGACAACTGATCTGGAGATATACTCGAGTATATCTCCAGATCAGTTGTC-3'
Other clones with same target sequence:
-
TRCN0000128272
