Construct: shRNA TRCN0000312570

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_005627.3-1510s21c1
DNA Barcode:
GCAATCTTATTGCACACTGTT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
SGK1 (6446)
Gene Description:
serum/glucocorticoid regulated kinase 1
Transcript:
RefSeq NM_005627.3 (NON-CURRENT)
Match location:
Position 1510 (3UTR)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000312570)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCAATCTTATTGCACACTGTT
Hairpin Sequence:
5'-CCGG-GCAATCTTATTGCACACTGTT-CTCGAG-AACAGTGTGCAATAAGATTGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCAATCTTATTGCACACTGTTCTCGAGAACAGTGTGCAATAAGATTGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCAATCTTATTGCACACTGTTCTCGAGAACAGTGTGCAATAAGATTGC-3'

Other clones with same target sequence:

  1. TRCN0000196562