Construct: shRNA TRCN0000312583

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_032875.1-1422s21c1
DNA Barcode:
AGGACCCATTTACCCAATATT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
FBXL20 (84961)
Gene Description:
F-box and leucine rich repeat protein 20
Transcript:
RefSeq NM_032875.1 (NON-CURRENT)
Match location:
Position 1422 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000312583)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
AGGACCCATTTACCCAATATT
Hairpin Sequence:
5'-CCGG-AGGACCCATTTACCCAATATT-CTCGAG-AATATTGGGTAAATGGGTCCT-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGAGGACCCATTTACCCAATATTCTCGAGAATATTGGGTAAATGGGTCCTTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAAGGACCCATTTACCCAATATTCTCGAGAATATTGGGTAAATGGGTCCT-3'

Other clones with same target sequence:

(none)