Construct: shRNA TRCN0000312592

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_020836.3-2157s21c1
DNA Barcode:
AGGGTCTTTCTTAACGCACTT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
BEGAIN (57596)
Gene Description:
brain enriched guanylate kinase associated
Transcript:
RefSeq NM_020836.3 (NON-CURRENT)
Match location:
Position 2157 (3UTR)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000312592)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
AGGGTCTTTCTTAACGCACTT
Hairpin Sequence:
5'-CCGG-AGGGTCTTTCTTAACGCACTT-CTCGAG-AAGTGCGTTAAGAAAGACCCT-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGAGGGTCTTTCTTAACGCACTTCTCGAGAAGTGCGTTAAGAAAGACCCTTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAAGGGTCTTTCTTAACGCACTTCTCGAGAAGTGCGTTAAGAAAGACCCT-3'

Other clones with same target sequence:

(none)