Construct: shRNA TRCN0000312597

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_004563.2-2065s21c1
DNA Barcode:
CTAGTCTAGCAAGAGGACATA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
PCK2 (5106)
Gene Description:
phosphoenolpyruvate carboxykinase 2, mitochondrial
Transcript:
RefSeq NM_004563.2 (NON-CURRENT)
Match location:
Position 2065 (3UTR)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000312597)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CTAGTCTAGCAAGAGGACATA
Hairpin Sequence:
5'-CCGG-CTAGTCTAGCAAGAGGACATA-CTCGAG-TATGTCCTCTTGCTAGACTAG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCTAGTCTAGCAAGAGGACATACTCGAGTATGTCCTCTTGCTAGACTAGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACTAGTCTAGCAAGAGGACATACTCGAGTATGTCCTCTTGCTAGACTAG-3'

Other clones with same target sequence:

(none)