Construct: shRNA TRCN0000312669
Construct Description:
- Construct Type:
- shRNA
- Other Identifiers:
- NM_005216.4-1695s21c1
- DNA Barcode:
- TTTCCACTTTGAATTGCTAAA
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- DDOST (1650)
- Gene Description:
- dolichyl-diphosphooligosaccharide--protein glycosyltransferase non-catalytic subunit
- Transcript:
- RefSeq NM_005216.4 (NON-CURRENT)
- Match location:
- Position 1695 (3UTR)
Vector Information:
- Vector Backbone:
- pLKO_005
- Pol II Cassette 1:
- PGK-PuroR
- Pol II Cassette 2:
- n/a
- Pol III Promoter:
- constitutive hU6
- Pol III Insert:
- (TRCN0000312669)
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
Current transcripts matched by this shRNA:
Sequence Information
- Target Sequence:
- TTTCCACTTTGAATTGCTAAA
- Hairpin Sequence:
-
5'-CCGG-TTTCCACTTTGAATTGCTAAA-CTCGAG-TTTAGCAATTCAAAGTGGAAA-TTTTTG-3'
Oligo design for arrayed cloning:
- Forward sequence:
- 5'-CCGGTTTCCACTTTGAATTGCTAAACTCGAGTTTAGCAATTCAAAGTGGAAATTTTTG-3'
- Reverse sequence:
- 5'-AATTCAAAAATTTCCACTTTGAATTGCTAAACTCGAGTTTAGCAATTCAAAGTGGAAA-3'
Other clones with same target sequence:
(none)
