Construct: shRNA TRCN0000312778

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_008636.4-1764s21c1
DNA Barcode:
GCCAACTCTGTCCTAACTAAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Mtf1 (17764)
Gene Description:
metal response element binding transcription factor 1
Transcript:
RefSeq NM_008636.4 (CURRENT)
Match location:
Position 1764 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000312778)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCCAACTCTGTCCTAACTAAT
Hairpin Sequence:
5'-CCGG-GCCAACTCTGTCCTAACTAAT-CTCGAG-ATTAGTTAGGACAGAGTTGGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCCAACTCTGTCCTAACTAATCTCGAGATTAGTTAGGACAGAGTTGGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCCAACTCTGTCCTAACTAATCTCGAGATTAGTTAGGACAGAGTTGGC-3'

Other clones with same target sequence:

  1. TRCN0000020456