Construct: shRNA TRCN0000312836

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_009210.2-430s21c1
DNA Barcode:
TTACAACGAGAGCCTAATAAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Hltf (20585)
Gene Description:
helicase-like transcription factor
Transcript:
RefSeq NM_009210.2 (NON-CURRENT)
Match location:
Position 430 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000312836)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
TTACAACGAGAGCCTAATAAT
Hairpin Sequence:
5'-CCGG-TTACAACGAGAGCCTAATAAT-CTCGAG-ATTATTAGGCTCTCGTTGTAA-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGTTACAACGAGAGCCTAATAATCTCGAGATTATTAGGCTCTCGTTGTAATTTTTG-3'
Reverse sequence:
5'-AATTCAAAAATTACAACGAGAGCCTAATAATCTCGAGATTATTAGGCTCTCGTTGTAA-3'

Other clones with same target sequence:

(none)