Construct: shRNA TRCN0000312891

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_145421.1-1034s21c1
DNA Barcode:
CATCGAGAAGGTGTCGAAGAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Abhd17a (216169)
Gene Description:
abhydrolase domain containing 17A
Transcript:
RefSeq NM_145421.1 (NON-CURRENT)
Match location:
Position 1034 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000312891)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CATCGAGAAGGTGTCGAAGAT
Hairpin Sequence:
5'-CCGG-CATCGAGAAGGTGTCGAAGAT-CTCGAG-ATCTTCGACACCTTCTCGATG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCATCGAGAAGGTGTCGAAGATCTCGAGATCTTCGACACCTTCTCGATGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACATCGAGAAGGTGTCGAAGATCTCGAGATCTTCGACACCTTCTCGATG-3'

Other clones with same target sequence:

(none)