Construct: shRNA TRCN0000313027

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_013825.3-2495s21c1
DNA Barcode:
ACTACGAAGAAGCCGTCTTAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Ly75 (17076)
Gene Description:
lymphocyte antigen 75
Transcript:
RefSeq NM_013825.3 (CURRENT)
Match location:
Position 2495 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000313027)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
ACTACGAAGAAGCCGTCTTAT
Hairpin Sequence:
5'-CCGG-ACTACGAAGAAGCCGTCTTAT-CTCGAG-ATAAGACGGCTTCTTCGTAGT-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGACTACGAAGAAGCCGTCTTATCTCGAGATAAGACGGCTTCTTCGTAGTTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAACTACGAAGAAGCCGTCTTATCTCGAGATAAGACGGCTTCTTCGTAGT-3'

Other clones with same target sequence:

(none)