Construct: shRNA TRCN0000313077

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_025482.2-106s21c1
DNA Barcode:
AGGTGTGCTGTCTGACTTTAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Tpd52l2 (66314)
Gene Description:
tumor protein D52-like 2
Transcript:
RefSeq NM_025482.2 (NON-CURRENT)
Match location:
Position 106 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000313077)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
AGGTGTGCTGTCTGACTTTAT
Hairpin Sequence:
5'-CCGG-AGGTGTGCTGTCTGACTTTAT-CTCGAG-ATAAAGTCAGACAGCACACCT-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGAGGTGTGCTGTCTGACTTTATCTCGAGATAAAGTCAGACAGCACACCTTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAAGGTGTGCTGTCTGACTTTATCTCGAGATAAAGTCAGACAGCACACCT-3'

Other clones with same target sequence:

(none)