Construct: shRNA TRCN0000313136

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_010065.2-2995s21c1
DNA Barcode:
CTCAGACTACCTGCTAGTTAC

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Dnm1 (13429)
Gene Description:
dynamin 1
Transcript:
RefSeq NM_010065.2 (NON-CURRENT)
Match location:
Position 2995 (3UTR)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000313136)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CTCAGACTACCTGCTAGTTAC
Hairpin Sequence:
5'-CCGG-CTCAGACTACCTGCTAGTTAC-CTCGAG-GTAACTAGCAGGTAGTCTGAG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCTCAGACTACCTGCTAGTTACCTCGAGGTAACTAGCAGGTAGTCTGAGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACTCAGACTACCTGCTAGTTACCTCGAGGTAACTAGCAGGTAGTCTGAG-3'

Other clones with same target sequence:

(none)