Construct: shRNA TRCN0000313210

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_172779.3-2974s21c1
DNA Barcode:
TTACTGAAGCCCTATAGTATG

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Ints6l (236790)
Gene Description:
integrator complex subunit 6 like
Transcript:
RefSeq NM_172779.3 (NON-CURRENT)
Match location:
Position 2974 (3UTR)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000313210)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
TTACTGAAGCCCTATAGTATG
Hairpin Sequence:
5'-CCGG-TTACTGAAGCCCTATAGTATG-CTCGAG-CATACTATAGGGCTTCAGTAA-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGTTACTGAAGCCCTATAGTATGCTCGAGCATACTATAGGGCTTCAGTAATTTTTG-3'
Reverse sequence:
5'-AATTCAAAAATTACTGAAGCCCTATAGTATGCTCGAGCATACTATAGGGCTTCAGTAA-3'

Other clones with same target sequence:

(none)