Construct: shRNA TRCN0000313294

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_138667.3-2133s21c1
DNA Barcode:
GGCGCCTGAAGAGATCAAATT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Tab2 (68652)
Gene Description:
TGF-beta activated kinase 1/MAP3K7 binding protein 2
Transcript:
RefSeq NM_138667.3 (CURRENT)
Match location:
Position 2133 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000313294)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GGCGCCTGAAGAGATCAAATT
Hairpin Sequence:
5'-CCGG-GGCGCCTGAAGAGATCAAATT-CTCGAG-AATTTGATCTCTTCAGGCGCC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGGCGCCTGAAGAGATCAAATTCTCGAGAATTTGATCTCTTCAGGCGCCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGGCGCCTGAAGAGATCAAATTCTCGAGAATTTGATCTCTTCAGGCGCC-3'

Other clones with same target sequence:

(none)