Construct: shRNA TRCN0000313386

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_026002.4-2288s21c1
DNA Barcode:
GAACGAGAATGATCGTAATTT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Mtdh (67154)
Gene Description:
metadherin
Transcript:
RefSeq NM_026002.4 (CURRENT)
Match location:
Position 2288 (3UTR)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000313386)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GAACGAGAATGATCGTAATTT
Hairpin Sequence:
5'-CCGG-GAACGAGAATGATCGTAATTT-CTCGAG-AAATTACGATCATTCTCGTTC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGAACGAGAATGATCGTAATTTCTCGAGAAATTACGATCATTCTCGTTCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGAACGAGAATGATCGTAATTTCTCGAGAAATTACGATCATTCTCGTTC-3'

Other clones with same target sequence:

(none)