Construct: shRNA TRCN0000313449

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_011394.3-919s21c1
DNA Barcode:
TTGTCGCCTCCTGGTTTATAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Slc20a2 (20516)
Gene Description:
solute carrier family 20, member 2
Transcript:
RefSeq NM_011394.3 (CURRENT)
Match location:
Position 919 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000313449)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
TTGTCGCCTCCTGGTTTATAT
Hairpin Sequence:
5'-CCGG-TTGTCGCCTCCTGGTTTATAT-CTCGAG-ATATAAACCAGGAGGCGACAA-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGTTGTCGCCTCCTGGTTTATATCTCGAGATATAAACCAGGAGGCGACAATTTTTG-3'
Reverse sequence:
5'-AATTCAAAAATTGTCGCCTCCTGGTTTATATCTCGAGATATAAACCAGGAGGCGACAA-3'

Other clones with same target sequence:

(none)