Construct: shRNA TRCN0000313455

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_026331.3-1599s21c1
DNA Barcode:
GTGCTTGTGTGTGTCTATAAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Slc25a37 (67712)
Gene Description:
solute carrier family 25, member 37
Transcript:
RefSeq NM_026331.3 (CURRENT)
Match location:
Position 1599 (3UTR)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000313455)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GTGCTTGTGTGTGTCTATAAA
Hairpin Sequence:
5'-CCGG-GTGCTTGTGTGTGTCTATAAA-CTCGAG-TTTATAGACACACACAAGCAC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGTGCTTGTGTGTGTCTATAAACTCGAGTTTATAGACACACACAAGCACTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGTGCTTGTGTGTGTCTATAAACTCGAGTTTATAGACACACACAAGCAC-3'

Other clones with same target sequence:

(none)