Construct: shRNA TRCN0000313515

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_201232.1-1642s21c1
DNA Barcode:
GGACGTAAACCGCCCACTTAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Nipbl (71175)
Gene Description:
Nipped-B homolog (Drosophila)
Transcript:
RefSeq NM_201232.1 (NON-CURRENT)
Match location:
Position 1642 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000313515)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GGACGTAAACCGCCCACTTAA
Hairpin Sequence:
5'-CCGG-GGACGTAAACCGCCCACTTAA-CTCGAG-TTAAGTGGGCGGTTTACGTCC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGGACGTAAACCGCCCACTTAACTCGAGTTAAGTGGGCGGTTTACGTCCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGGACGTAAACCGCCCACTTAACTCGAGTTAAGTGGGCGGTTTACGTCC-3'

Other clones with same target sequence:

(none)