Construct: shRNA TRCN0000313624

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_015819.3-1001s21c1
DNA Barcode:
TCGCCGTTTACCCAGTATAAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Hs6st2 (50786)
Gene Description:
heparan sulfate 6-O-sulfotransferase 2
Transcript:
RefSeq NM_015819.3 (NON-CURRENT)
Match location:
Position 1001 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000313624)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
TCGCCGTTTACCCAGTATAAT
Hairpin Sequence:
5'-CCGG-TCGCCGTTTACCCAGTATAAT-CTCGAG-ATTATACTGGGTAAACGGCGA-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGTCGCCGTTTACCCAGTATAATCTCGAGATTATACTGGGTAAACGGCGATTTTTG-3'
Reverse sequence:
5'-AATTCAAAAATCGCCGTTTACCCAGTATAATCTCGAGATTATACTGGGTAAACGGCGA-3'

Other clones with same target sequence:

(none)