Construct: shRNA TRCN0000313678

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_011303.5-1637s21c1
DNA Barcode:
GCCTGATGTGCATCTACTATT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Dhrs3 (20148)
Gene Description:
dehydrogenase/reductase (SDR family) member 3
Transcript:
RefSeq NM_011303.5 (NON-CURRENT)
Match location:
Position 1637 (3UTR)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000313678)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCCTGATGTGCATCTACTATT
Hairpin Sequence:
5'-CCGG-GCCTGATGTGCATCTACTATT-CTCGAG-AATAGTAGATGCACATCAGGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCCTGATGTGCATCTACTATTCTCGAGAATAGTAGATGCACATCAGGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCCTGATGTGCATCTACTATTCTCGAGAATAGTAGATGCACATCAGGC-3'

Other clones with same target sequence:

(none)