Construct: shRNA TRCN0000313719

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_138604.2-1075s21c1
DNA Barcode:
AGAAGACTTCACCACCTATAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Otud5 (54644)
Gene Description:
OTU domain containing 5
Transcript:
RefSeq NM_138604.2 (NON-CURRENT)
Match location:
Position 1075 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000313719)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
AGAAGACTTCACCACCTATAT
Hairpin Sequence:
5'-CCGG-AGAAGACTTCACCACCTATAT-CTCGAG-ATATAGGTGGTGAAGTCTTCT-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGAGAAGACTTCACCACCTATATCTCGAGATATAGGTGGTGAAGTCTTCTTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAAGAAGACTTCACCACCTATATCTCGAGATATAGGTGGTGAAGTCTTCT-3'

Other clones with same target sequence:

(none)