Construct: shRNA TRCN0000313887

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_013882.1-205s21c1
DNA Barcode:
TTGGGCCTGTTGGACATAAAG

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Gtse1 (29870)
Gene Description:
G two S phase expressed protein 1
Transcript:
RefSeq NM_013882.1 (NON-CURRENT)
Match location:
Position 205 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000313887)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
TTGGGCCTGTTGGACATAAAG
Hairpin Sequence:
5'-CCGG-TTGGGCCTGTTGGACATAAAG-CTCGAG-CTTTATGTCCAACAGGCCCAA-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGTTGGGCCTGTTGGACATAAAGCTCGAGCTTTATGTCCAACAGGCCCAATTTTTG-3'
Reverse sequence:
5'-AATTCAAAAATTGGGCCTGTTGGACATAAAGCTCGAGCTTTATGTCCAACAGGCCCAA-3'

Other clones with same target sequence:

(none)