Construct: shRNA TRCN0000313905

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_133701.2-1120s21c1
DNA Barcode:
AGTAGCTCGAAACCTTATTAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Prpf6 (68879)
Gene Description:
pre-mRNA splicing factor 6
Transcript:
RefSeq NM_133701.2 (CURRENT)
Match location:
Position 1120 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000313905)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
AGTAGCTCGAAACCTTATTAT
Hairpin Sequence:
5'-CCGG-AGTAGCTCGAAACCTTATTAT-CTCGAG-ATAATAAGGTTTCGAGCTACT-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGAGTAGCTCGAAACCTTATTATCTCGAGATAATAAGGTTTCGAGCTACTTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAAGTAGCTCGAAACCTTATTATCTCGAGATAATAAGGTTTCGAGCTACT-3'

Other clones with same target sequence:

(none)