Construct: shRNA TRCN0000314037

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_145355.4-843s21c1
DNA Barcode:
TGGCTCCTGATCGCCTAATAC

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Rnf185 (193670)
Gene Description:
ring finger protein 185
Transcript:
RefSeq NM_145355.4 (NON-CURRENT)
Match location:
Position 843 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000314037)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
TGGCTCCTGATCGCCTAATAC
Hairpin Sequence:
5'-CCGG-TGGCTCCTGATCGCCTAATAC-CTCGAG-GTATTAGGCGATCAGGAGCCA-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGTGGCTCCTGATCGCCTAATACCTCGAGGTATTAGGCGATCAGGAGCCATTTTTG-3'
Reverse sequence:
5'-AATTCAAAAATGGCTCCTGATCGCCTAATACCTCGAGGTATTAGGCGATCAGGAGCCA-3'

Other clones with same target sequence:

(none)