Construct: shRNA TRCN0000314113

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_011285.1-1257s21c1
DNA Barcode:
CCTAAATTTGAAGACGTATAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Rpgr (19893)
Gene Description:
retinitis pigmentosa GTPase regulator
Transcript:
RefSeq NM_011285.1 (NON-CURRENT)
Match location:
Position 1257 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000314113)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCTAAATTTGAAGACGTATAT
Hairpin Sequence:
5'-CCGG-CCTAAATTTGAAGACGTATAT-CTCGAG-ATATACGTCTTCAAATTTAGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCTAAATTTGAAGACGTATATCTCGAGATATACGTCTTCAAATTTAGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCTAAATTTGAAGACGTATATCTCGAGATATACGTCTTCAAATTTAGG-3'

Other clones with same target sequence:

(none)