Construct: shRNA TRCN0000314116

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_016769.3-1592s21c1
DNA Barcode:
TTAGAGACACTAGGAGTAAAG

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Smad3 (17127)
Gene Description:
SMAD family member 3
Transcript:
RefSeq NM_016769.3 (NON-CURRENT)
Match location:
Position 1592 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000314116)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
TTAGAGACACTAGGAGTAAAG
Hairpin Sequence:
5'-CCGG-TTAGAGACACTAGGAGTAAAG-CTCGAG-CTTTACTCCTAGTGTCTCTAA-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGTTAGAGACACTAGGAGTAAAGCTCGAGCTTTACTCCTAGTGTCTCTAATTTTTG-3'
Reverse sequence:
5'-AATTCAAAAATTAGAGACACTAGGAGTAAAGCTCGAGCTTTACTCCTAGTGTCTCTAA-3'

Other clones with same target sequence:

(none)