Construct: shRNA TRCN0000314141

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_008872.2-568s21c1
DNA Barcode:
TGTCGCTGAAGCCCTACAATG

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Plat (18791)
Gene Description:
plasminogen activator, tissue
Transcript:
RefSeq NM_008872.2 (NON-CURRENT)
Match location:
Position 568 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000314141)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
TGTCGCTGAAGCCCTACAATG
Hairpin Sequence:
5'-CCGG-TGTCGCTGAAGCCCTACAATG-CTCGAG-CATTGTAGGGCTTCAGCGACA-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGTGTCGCTGAAGCCCTACAATGCTCGAGCATTGTAGGGCTTCAGCGACATTTTTG-3'
Reverse sequence:
5'-AATTCAAAAATGTCGCTGAAGCCCTACAATGCTCGAGCATTGTAGGGCTTCAGCGACA-3'

Other clones with same target sequence:

(none)