Construct: shRNA TRCN0000314229

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_025387.3-383s21c1
DNA Barcode:
CCTAGCTACATCTGGGACCTT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Tmem14c (66154)
Gene Description:
transmembrane protein 14C
Transcript:
RefSeq NM_025387.3 (CURRENT)
Match location:
Position 383 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000314229)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCTAGCTACATCTGGGACCTT
Hairpin Sequence:
5'-CCGG-CCTAGCTACATCTGGGACCTT-CTCGAG-AAGGTCCCAGATGTAGCTAGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCTAGCTACATCTGGGACCTTCTCGAGAAGGTCCCAGATGTAGCTAGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCTAGCTACATCTGGGACCTTCTCGAGAAGGTCCCAGATGTAGCTAGG-3'

Other clones with same target sequence:

  1. TRCN0000009767