Construct: shRNA TRCN0000314289

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_009291.1-1091s21c1
DNA Barcode:
GAGAAGTATCTGAGAACTCTT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Stra6 (20897)
Gene Description:
stimulated by retinoic acid gene 6
Transcript:
RefSeq NM_009291.1 (NON-CURRENT)
Match location:
Position 1091 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000314289)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GAGAAGTATCTGAGAACTCTT
Hairpin Sequence:
5'-CCGG-GAGAAGTATCTGAGAACTCTT-CTCGAG-AAGAGTTCTCAGATACTTCTC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGAGAAGTATCTGAGAACTCTTCTCGAGAAGAGTTCTCAGATACTTCTCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGAGAAGTATCTGAGAACTCTTCTCGAGAAGAGTTCTCAGATACTTCTC-3'

Other clones with same target sequence:

  1. TRCN0000175720