Construct: shRNA TRCN0000314314

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_133891.3-565s21c1
DNA Barcode:
CCTGGACTTGATAAACAGGAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Slc44a1 (100434)
Gene Description:
solute carrier family 44, member 1
Transcript:
RefSeq NM_133891.3 (CURRENT)
Match location:
Position 565 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000314314)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCTGGACTTGATAAACAGGAA
Hairpin Sequence:
5'-CCGG-CCTGGACTTGATAAACAGGAA-CTCGAG-TTCCTGTTTATCAAGTCCAGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCTGGACTTGATAAACAGGAACTCGAGTTCCTGTTTATCAAGTCCAGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCTGGACTTGATAAACAGGAACTCGAGTTCCTGTTTATCAAGTCCAGG-3'

Other clones with same target sequence:

  1. TRCN0000102054