Construct: shRNA TRCN0000314425

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_025697.3-1296s21c1
DNA Barcode:
CCTCTGTTAATGGACTACTTT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Spryd7 (66674)
Gene Description:
SPRY domain containing 7
Transcript:
RefSeq NM_025697.3 (NON-CURRENT)
Match location:
Position 1296 (3UTR)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000314425)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCTCTGTTAATGGACTACTTT
Hairpin Sequence:
5'-CCGG-CCTCTGTTAATGGACTACTTT-CTCGAG-AAAGTAGTCCATTAACAGAGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCTCTGTTAATGGACTACTTTCTCGAGAAAGTAGTCCATTAACAGAGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCTCTGTTAATGGACTACTTTCTCGAGAAAGTAGTCCATTAACAGAGG-3'

Other clones with same target sequence:

  1. TRCN0000191304