Construct: shRNA TRCN0000314509

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_006511.1-1097s21c1
DNA Barcode:
GCAGTAAACCAGCTTCAGAAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
RSC1A1 (6248)
Gene Description:
regulator of solute carriers 1
Transcript:
RefSeq NM_006511.1 (NON-CURRENT)
Match location:
Position 1097 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000314509)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCAGTAAACCAGCTTCAGAAA
Hairpin Sequence:
5'-CCGG-GCAGTAAACCAGCTTCAGAAA-CTCGAG-TTTCTGAAGCTGGTTTACTGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCAGTAAACCAGCTTCAGAAACTCGAGTTTCTGAAGCTGGTTTACTGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCAGTAAACCAGCTTCAGAAACTCGAGTTTCTGAAGCTGGTTTACTGC-3'

Other clones with same target sequence:

  1. TRCN0000002946