Construct: shRNA TRCN0000314514

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_139168.2-2196s21c1
DNA Barcode:
AGTGTACCTCTGCACTCAATG

Original Target:

Taxon:
Homo sapiens (human)
Gene:
SREK1 (140890)
Gene Description:
splicing regulatory glutamic acid and lysine rich protein 1
Transcript:
RefSeq NM_139168.2 (NON-CURRENT)
Match location:
Position 2196 (3UTR)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000314514)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
AGTGTACCTCTGCACTCAATG
Hairpin Sequence:
5'-CCGG-AGTGTACCTCTGCACTCAATG-CTCGAG-CATTGAGTGCAGAGGTACACT-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGAGTGTACCTCTGCACTCAATGCTCGAGCATTGAGTGCAGAGGTACACTTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAAGTGTACCTCTGCACTCAATGCTCGAGCATTGAGTGCAGAGGTACACT-3'

Other clones with same target sequence:

(none)