Construct: shRNA TRCN0000314546

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_002675.3-492s21c1
DNA Barcode:
GTGTACCGGCAGATTGTGGAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
PML (5371)
Gene Description:
promyelocytic leukemia
Transcript:
RefSeq NM_002675.3 (CURRENT)
Match location:
Position 492 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000314546)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GTGTACCGGCAGATTGTGGAT
Hairpin Sequence:
5'-CCGG-GTGTACCGGCAGATTGTGGAT-CTCGAG-ATCCACAATCTGCCGGTACAC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGTGTACCGGCAGATTGTGGATCTCGAGATCCACAATCTGCCGGTACACTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGTGTACCGGCAGATTGTGGATCTCGAGATCCACAATCTGCCGGTACAC-3'

Other clones with same target sequence:

  1. TRCN0000003868