Construct: shRNA TRCN0000314575

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_001357.3-3663s21c1
DNA Barcode:
ACGACAATGGAAGCGGATATA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
DHX9 (1660)
Gene Description:
DExH-box helicase 9
Transcript:
RefSeq NM_001357.3 (NON-CURRENT)
Match location:
Position 3663 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000314575)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
ACGACAATGGAAGCGGATATA
Hairpin Sequence:
5'-CCGG-ACGACAATGGAAGCGGATATA-CTCGAG-TATATCCGCTTCCATTGTCGT-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGACGACAATGGAAGCGGATATACTCGAGTATATCCGCTTCCATTGTCGTTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAACGACAATGGAAGCGGATATACTCGAGTATATCCGCTTCCATTGTCGT-3'

Other clones with same target sequence:

(none)